Taify Pomegranate Juice Extract Abrogates Testicular Dysfunction Induced by Acrylamide: Role of Inflammatory, Antioxidants, and Oxidative Stress Biomarkers

High altitude (HA) poses various dangers to living organisms, the most serious of which are oxidative stress and its accompanying metabolic problems, as well hypoxia associated abnormalities. Acrylamide is a poisonous chemical produced under result intracellular reactive oxygen species formation toxicity. Heating carbohydrates-rich meals give acrylamide that widely used in industry. The precise mechanism toxicity unknown. current study aimed examine impacts Taify pomegranate juice (TPJ) from Taif area (Punica granatum L.) on acrylamide-induced testicular dysfunction. Twenty four male adult Wistar rats, were divided into groups: Group 1 was negative-control received only saline; 2 positive-control orally (20 mg/kg/bw) for consecutive 4 weeks; 3 TPJ; mL/kg/bw, weeks. Rats described Groups 3, with TPJ delivered week before acrylamide. elevated serum levels inflammatory cytokines (interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α)). Moreover, decreased concentrations follicle stimulating hormone (FSH), leutinizing (LH), testosterone altered semen characteristics (decreased sperm viability concentration, increased abnormalities). malondialdehyde (MDA), while it antioxidant activities (superoxide dismutase; SOD reduced glutathione; GSH). inflammation restored by administration rats. GSH significantly recovered near control plus group compared acrylamide-administered preadministration rats profiles, alteration testis pathology, normalized changes reproductive hormones affected Furthermore, reversed upregulation caspases-3 decrease B-cell lymphoma-2 (Bcl-2) gene expressions acrylamide, significant hemoxygenase-1 (HO-1) nuclear erythroid-2 (Nrf2) mRNA expression. These findings collectively revealed possesses anti-inflammatory, potent antioxidant, antiapoptotic effect against damage.